In vitro direct plant regeneration from shoot tip explants of Clitoria ternatea - An important medicinal plant

A simple micropropagation method from shoot tip explants was reported here for Clitoria ternatea L., an economically important medicinal plant. High frequency of micro shoots were obtained from this explants on MS medium supplemented with various concentrations of Indole Acetic acid (IAA) (0.5 2.0 mg/l) and KN (0.5 -2.0 mg/l). Maximum number of shoots was obtained from shoot tip explants in the medium containing 0.5 mg/l IAA and 1.0 mg/l Kinetin (KN). The regenerated shoots were further elongated on MS medium supplemented with IAA (0.10.6 mg/l) and 6-Benzylaminopurine (BAP) (0.1 0.6 mg/l). Among these concentrations, high length of shoot elongation was achieved in the medium containing BAP (0.5 mg/l) and IAA (0.5 mg/l).